Posted by Thermoline Staff on Mar 22nd 2017
Centrifugation is one of the most widely used laboratory techniques for the separation of materials in the fields of biochemistry, molecular biology, medicine, food sciences and industry. It’s all about gravity and mass: particles in a heterogeneous solution will, given enough time, separate based on their size and density. Smaller, less-dense particles may also migrate down, but not always; some particles will never settle, but remain suspended in solution. Centrifuges force this process along much more quickly and efficiently.
When getting enquiries for laboratory centrifuges, we are often asked what the difference is between a swing out rotor and a fixed angle rotor. Both types of rotor serve a purpose and both have advantages and disadvantages over the other. Let’s have a look at what a swing angle rotor and fixed angle rotor is.
Swing Out Rotor
Swing out rotor (also known as bucket rotor, rotor with variable angle) is the rotor, in which tubes are located in the buckets (tube-holders) and those buckets are not rigidly attached to the rotor with certain angle. Instead buckets are attached to the rotor in a way, that allows them to spin horizontally (angle relative to the axis of rotation - 90 degrees). This rotor is particularly useful when samples are to be resolved in density gradients. The longer path length permits better separation of individual particle types from a mixture. However, this rotor is relatively inefficient for pelleting.
Benefits of a swing out bucket rotor
Fixed Angle Rotor
Fixed angle rotor – is the rotor, which holds tubes in the exact same fixed angle all the time, usually 45 degrees. When the rotor begins to rotate, the solution in the tubes reorients. This rotor type is most commonly used for pelleting applications. Examples include pelleting bacteria, yeast, and other mammalian cells. It is also useful for isopycnic separations of macromolecules such as nucleic acids.
Recall that more dense materials will separate towards the angle of the centripetal force. Therefore, in swinging-bucket rotors, the pelleted material will form at the bottom of the conical centrifuge tube, while tubes processed in a fixed-angle rotor will form sedimentation on the side. This could be problematic if the solids get caught in the angle of the tube.
All things being equal, the fact that swinging-bucket rotors offer a superior sedimentation location makes them the obvious choice for centrifuging samples; however, fixed-angle rotors offer valuable features that often make them a more desirable choice.
Benefits of a fixed angle rotor
Choosing the right rotor for you
To assist us in choosing the right rotor for you we require the following:
Appropriate buckets and the correct adaptors which suit your particular tubes can then be selected for swing out rotors. Sealing lids are also available to seal the tubes inside the bucket. High speed fixed angle rotors are available and are manufactured from aircraft grade alloy to withstand the higher speeds.
To get more information, please visit the centrifuges section of our website at this link: http://www.thermoline.com.au/products/laboratory-e...
Thermoline Scientific will be hosting a series of informative seminars presented by Rich Kish, International Sales Manager for Q-Lab.Seminar from 9am to 3pm with morning tea from 8:30am.Sydney on 14th FebruaryRydges RosehillExecutive Boardroom116-118 James Ruse Dr, Rosehill* Free parking onsite.Melbourne on 16th FebruaryRydges on SwanstonBoardroom Three701 Swanston St, Carlton* Full day parking on site $25 [...]
Unfortunately, it’s not uncommon to see domestic style refrigerators being used to store vaccines and drugs in chemists, hospitals and even at your local GP. There can be serious implications with doing so as domestic style refrigerators are not designed to handle such critical product. There are many disadvantages when it comes to storing vaccines, [...]
December 21st brings the end to two long standing and highly regarded employees at Thermoline Scientific. Charlie arrived at Thermoline some 28 years ago and has been leading the production section for most of that.Greg arrived 22 and a bit years ago and has been the factory foreman and sheetmetal leader for most of that [...]
To our valued customers,We would like to wish you, your staff and families a Merry Christmas and Happy New Year. Thank you for your continued support throughout 2016 and we look forward to working with you again in 2017.Christmas Closure Dates: 21st December (last despatch 20th Dec). Our office will reopen on Monday the 16th of [...]
If coatings tests are to be reliable and reproducible, they must be performed on a substrate which is reasonably consistent from test to test. Unfortunately, ordinary commercial sheet steel displays wide variations in the surface properties which affect the bonding of coatings. Sources of Variability Surface problems can come from a number of sources: Surface [...]
In September 2016, Thermoline Scientific were proud to exhibit at the 5th International Controlled Environment Conference / AusPheno 2016. This was a great opportunity for the Australian and International plant science community to come together over a week of talks and activities held at the CSIRO Discovery Centre in Canberra.On display we had a 520L [...]
Thermoline were invited to exhibit at the 56th annual Surface Coatings Association Australia Conference held early September 2016 at the Pullman & Mercure conference Centre Albert Park Melbourne.This was a perfect opportunity for us to exhibit our NEW range of accelerated weathering test cabinets manufactured by Q-Lab in the USA, and a fantastic opportunity to meet face to face and [...]
Thermoline is proud to have available a budget friendly, high quality range of environmental testing chambers available in 5 sizes from 150L up to 1000L. KOMEG Technology has been manufacturing environmental test equipment since 1990 and specialises in climatic test chambers, thermal shock test chambers, walk-in chambers and medical laboratory equipment. Product Key Features: Five sizes available starting [...]
Thermoline's range of EasyLog data loggers are a cost effective and easy-to-use method of recording temperature and humidity data ranges on your Thermoline products. Manufactured by Lascar, the EL-USB loggers are the world's first direct USB data loggers with several models also featuring WiFi connectivity. All loggers are compact and light weight so they can [...]